Preparation and characterization of new WHO reference reagents for human chorionic gonadotropin and metabolites
Identifieur interne : 00B074 ( Main/Exploration ); précédent : 00B073; suivant : 00B075Preparation and characterization of new WHO reference reagents for human chorionic gonadotropin and metabolites
Auteurs : Steven Birken [États-Unis] ; Peter Berger [Autriche] ; Jean-Michel Bidart [France] ; Matthias Weber [Allemagne] ; Adrian Bristow [Royaume-Uni] ; Rob Norman [Australie] ; Catharine Sturgeon [Royaume-Uni] ; Ulf-Hakan Stenman [Finlande]Source :
- Clinical chemistry : (Baltimore, Md.) [ 0009-9147 ] ; 2003.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Homme, Normalisation.
English descriptors
- KwdEn :
Abstract
Background: The currently used standards for human chorionic gonadotropin (hCG) and its a and β subunits (hCGa and hCGβ) contain substantial amounts of contaminating variants of hCG and other impurities. Furthermore, some partially degraded forms of hCG and its subunits have become of potential clinical importance, e.g., "nicked" forms of hCG (hCGn) and hCGβ (hCGβn), which contain cuts in the peptide backbone between amino acids 44-45 or 47-48 in hCGβ, and a fragment of hCGβ (hCGβcf) consisting of amino acids 6-40 and 55-92 bound together by disulfide bridges. The IFCC appointed a working group with the aim of preparing new standards for hCG and related substances to improve standardization of their immunoassays. Methods: Large amounts of hCG and its subunits as well as of hCGn, hCGβn, and hCGβcf were prepared by previously developed purification methods in combination with hydrophobic interaction chromatography and reversed-phase HPLC. Each preparation was characterized on the basis of amino acid and sequence analyses, carbohydrate composition, and electrophoretic patterns. Immunoassays for relevant contaminating proteins were also performed. Results: The major preparations were homogeneous and free of contaminating proteins. Concentrations of the final preparations were determined by amino acid analysis. Conclusions: Calibrated in substance concentrations (mol/L) based on amino acid analyses, these preparations will facilitate improved standardization of immunoassays for hCG and its metabolites. The six preparations have now been established by the WHO as new 1st Reference Reagents for immunoassays with the following codes: hCG 99/688, hCGβ 99/650, hCGa 99/720, hCGn 99/642, hCGβn 99/692, and hCGβcf 99/708. In contrast to the 3rd International Standard (75/537), the clinically most important Reference Reagent for hCG (99/688) contains no hCGn and negligible amounts of free subunits.
Affiliations:
- Allemagne, Australie, Autriche, Finlande, France, Royaume-Uni, États-Unis
- Bavière, District de Haute-Bavière
- Munich
- Université Louis-et-Maximilien de Munich
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reference reagents for human chorionic gonadotropin and metabolites</title><author><name sortKey="Birken, Steven" sort="Birken, Steven" uniqKey="Birken S" first="Steven" last="Birken">Steven Birken</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>College of Physicians and Surgeons of Columbia University</s1><s2>New York, NY 10032</s2><s3>USA</s3><sZ>1 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>College of Physicians and Surgeons of Columbia University</wicri:noRegion></affiliation></author><author><name sortKey="Berger, Peter" sort="Berger, Peter" uniqKey="Berger P" first="Peter" last="Berger">Peter Berger</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Institute for Biomedical Aging Research, Austrian Academy of Sciences</s1><s2>6020 Innsbruck</s2><s3>AUT</s3><sZ>2 aut.</sZ></inist:fA14><country>Autriche</country><wicri:noRegion>6020 Innsbruck</wicri:noRegion></affiliation></author><author><name sortKey="Bidart, Jean Michel" sort="Bidart, Jean Michel" uniqKey="Bidart J" first="Jean-Michel" last="Bidart">Jean-Michel Bidart</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Clinical Biology, Institut Gustave-Roussy</s1><s2>94805 Villejuif</s2><s3>FRA</s3><sZ>3 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>94805 Villejuif</wicri:noRegion><wicri:noRegion>Institut Gustave-Roussy</wicri:noRegion><wicri:noRegion>94805 Villejuif</wicri:noRegion></affiliation></author><author><name sortKey="Weber, Matthias" sort="Weber, Matthias" uniqKey="Weber M" first="Matthias" last="Weber">Matthias Weber</name><affiliation wicri:level="4"><inist:fA14 i1="04"><s1>Klinikum Grosshadern, University of Munich</s1><s2>81377 Munich</s2><s3>DEU</s3><sZ>4 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Haute-Bavière</region><settlement type="city">Munich</settlement><settlement type="city">Munich</settlement></placeName><orgName type="university">Université Louis-et-Maximilien de Munich</orgName></affiliation></author><author><name sortKey="Bristow, Adrian" sort="Bristow, Adrian" uniqKey="Bristow A" first="Adrian" last="Bristow">Adrian Bristow</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>National Institute of Biological Standards and Control</s1><s2>Potters Bar, Herts EN6 3QG</s2><s3>GBR</s3><sZ>5 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>National Institute of Biological Standards and Control</wicri:noRegion></affiliation></author><author><name sortKey="Norman, Rob" sort="Norman, Rob" uniqKey="Norman R" first="Rob" last="Norman">Rob Norman</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Reproductive Medicine Unit, Department of Obstetrics and Gynaecology, University of Adelaide, The Queen Elizabeth Hospital</s1><s2>Woodville, South Australia 5011</s2><s3>AUS</s3><sZ>6 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Woodville, South Australia 5011</wicri:noRegion></affiliation></author><author><name sortKey="Sturgeon, Catharine" sort="Sturgeon, Catharine" uniqKey="Sturgeon C" first="Catharine" last="Sturgeon">Catharine Sturgeon</name><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Department of Clinical Biochemistry, Royal Infirmary</s1><s2>Edinburgh EH3 9YW</s2><s3>GBR</s3><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Edinburgh EH3 9YW</wicri:noRegion></affiliation></author><author><name sortKey="Stenman, Ulf Hakan" sort="Stenman, Ulf Hakan" uniqKey="Stenman U" first="Ulf-Hakan" last="Stenman">Ulf-Hakan Stenman</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Department of Clinical Chemistry, Helsinki University Central Hospital</s1><s2>Helsinki 00290</s2><s3>FIN</s3><sZ>8 aut.</sZ></inist:fA14><country>Finlande</country><wicri:noRegion>Helsinki 00290</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title><title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title><idno type="ISSN">0009-9147</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Clinical chemistry : (Baltimore, Md.)</title><title level="j" type="abbreviated">Clin. chem. : (Baltim. Md.)</title><idno type="ISSN">0009-9147</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Analytical standard</term><term>Exploration</term><term>HPLC chromatography</term><term>Human</term><term>Human chorionic gonadotrophin</term><term>Hydrophobic chromatography</term><term>Immunological method</term><term>Metabolite</term><term>Pre analytical step</term><term>Preparation</term><term>Purification</term><term>Reagents</term><term>Standardization</term><term>WHO</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>HCG</term><term>Exploration</term><term>Homme</term><term>Normalisation</term><term>Réactif</term><term>Préparation</term><term>Phase préanalytique</term><term>Métabolite</term><term>OMS</term><term>Méthode immunologique</term><term>Purification</term><term>Chromatographie hydrophobe</term><term>Chromatographie HPLC</term><term>Etalon analytique</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term><term>Normalisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: The currently used standards for human chorionic gonadotropin (hCG) and its a and β subunits (hCGa and hCGβ) contain substantial amounts of contaminating variants of hCG and other impurities. Furthermore, some partially degraded forms of hCG and its subunits have become of potential clinical importance, e.g., "nicked" forms of hCG (hCGn) and hCGβ (hCGβn), which contain cuts in the peptide backbone between amino acids 44-45 or 47-48 in hCGβ, and a fragment of hCGβ (hCGβcf) consisting of amino acids 6-40 and 55-92 bound together by disulfide bridges. The IFCC appointed a working group with the aim of preparing new standards for hCG and related substances to improve standardization of their immunoassays. Methods: Large amounts of hCG and its subunits as well as of hCGn, hCGβn, and hCGβcf were prepared by previously developed purification methods in combination with hydrophobic interaction chromatography and reversed-phase HPLC. Each preparation was characterized on the basis of amino acid and sequence analyses, carbohydrate composition, and electrophoretic patterns. Immunoassays for relevant contaminating proteins were also performed. Results: The major preparations were homogeneous and free of contaminating proteins. Concentrations of the final preparations were determined by amino acid analysis. Conclusions: Calibrated in substance concentrations (mol/L) based on amino acid analyses, these preparations will facilitate improved standardization of immunoassays for hCG and its metabolites. The six preparations have now been established by the WHO as new 1st Reference Reagents for immunoassays with the following codes: hCG 99/688, hCGβ 99/650, hCGa 99/720, hCGn 99/642, hCGβn 99/692, and hCGβcf 99/708. In contrast to the 3rd International Standard (75/537), the clinically most important Reference Reagent for hCG (99/688) contains no hCGn and negligible amounts of free subunits.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Australie</li><li>Autriche</li><li>Finlande</li><li>France</li><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Bavière</li><li>District de Haute-Bavière</li></region><settlement><li>Munich</li></settlement><orgName><li>Université Louis-et-Maximilien de Munich</li></orgName></list><tree><country name="États-Unis"><noRegion><name sortKey="Birken, Steven" sort="Birken, Steven" uniqKey="Birken S" first="Steven" last="Birken">Steven Birken</name></noRegion></country><country name="Autriche"><noRegion><name sortKey="Berger, Peter" sort="Berger, Peter" uniqKey="Berger P" first="Peter" last="Berger">Peter Berger</name></noRegion></country><country name="France"><noRegion><name sortKey="Bidart, Jean Michel" sort="Bidart, Jean Michel" uniqKey="Bidart J" first="Jean-Michel" last="Bidart">Jean-Michel Bidart</name></noRegion></country><country name="Allemagne"><region name="Bavière"><name sortKey="Weber, Matthias" sort="Weber, Matthias" uniqKey="Weber M" first="Matthias" last="Weber">Matthias Weber</name></region></country><country name="Royaume-Uni"><noRegion><name sortKey="Bristow, Adrian" sort="Bristow, Adrian" uniqKey="Bristow A" first="Adrian" last="Bristow">Adrian Bristow</name></noRegion><name sortKey="Sturgeon, Catharine" sort="Sturgeon, Catharine" uniqKey="Sturgeon C" first="Catharine" last="Sturgeon">Catharine Sturgeon</name></country><country name="Australie"><noRegion><name sortKey="Norman, Rob" sort="Norman, Rob" uniqKey="Norman R" first="Rob" last="Norman">Rob Norman</name></noRegion></country><country name="Finlande"><noRegion><name sortKey="Stenman, Ulf Hakan" sort="Stenman, Ulf Hakan" uniqKey="Stenman U" first="Ulf-Hakan" last="Stenman">Ulf-Hakan Stenman</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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